Matt Lycas

Quantitative Expansion Microscopy

Applying quantitative imaging to expansion microscopy to achieve in situ estimation of protein abundance.

Cryo EM Tomography of Dopaminergic Synapses

Observing pharmacological changes to the ultrastructure of the dopaminergic presynapse using advanced cryo-electron microscopy techniques.

Live STED for Mitochondria Cristae Dynamics

Use of these tools to help examine the role of cristae in mitochondrial pearling and understand dynamic organelle behavior.

Manuscript in Preparation (2025)

Title: "Quantitative Expansion Microscopy for In Situ Estimation of Endogenous Target Abundance"

Authors: Lycas, M.D., Landoni, J.C., Noferi, B., Zimmerli, C.E., Douglass, K.M., Manley, S.

Lead author - Developing quantitative approaches for expansion microscopy to estimate protein abundance in situ.

bioRxiv (2024)

Title: "Ultrastructural Dynamics of Dopaminergic Presynaptic Release Sites revealed by Cryo-correlative Light and Electron Microscopy"

Authors: Lycas, M.D., Morado, D.R., Gether, U., Briggs, J.A.G., Erlendsson, S.

DOI: 2024.04.15.589543

Lead author - Investigating dopaminergic synapse ultrastructure using advanced cryo-EM techniques.

bioRxiv (2024)

Title: "Pearling Drives Mitochondrial DNA Nucleoid Distribution"

Authors: Landoni, J.C., Lycas, M.D., Macuada, J., Stepp, W., Jaccard, R., Obara, C.J., Moore, A.S., Hoffman, D., Lippincott-Schwartz, J., Marshall, W., Sturm, G., Manley, S.

DOI: 2024.12.21.629917

Co-author - Live STED imaging to exploring mitochondrial dynamics and DNA distribution through pearling mechanisms.